QTL-seq for identification of loci associated with resistance to Phytophthora crown rot in squash.
Identifieur interne : 000079 ( Main/Exploration ); précédent : 000078; suivant : 000080QTL-seq for identification of loci associated with resistance to Phytophthora crown rot in squash.
Auteurs : Alexis Ramos [États-Unis] ; Yuqing Fu [États-Unis] ; Vincent Michael [États-Unis] ; Geoffrey Meru [États-Unis]Source :
- Scientific reports [ 2045-2322 ] ; 2020.
Abstract
Phytophthora capsici Leonian, the causal agent of foliar blight, root rot, fruit rot and crown rot syndromes in squash (Cucurbita moschata), is a devastating pathogen worldwide. Resistance to Phytophthora crown rot in University of Florida breeding line #394-1-27-12 (C. moschata) is conferred by three independent dominant genes (R1R2R3). Availability of DNA markers linked to R1R2R3 genes would allow efficient breeding for Phytophthora crown rot resistance through marker-assisted selection (MAS). The goal of the current study was to identify quantitative trait loci (QTLs) associated with resistance to Phytophthora crown rot in an F2 population (n = 168) derived from a cross between #394-1-27-12 (R) and Butter Bush (S) using QTL-seq bulk segregant analysis. Whole-genome resequencing of the resistant (n = 20) and susceptible (n = 20) bulk segregants revealed ~900,000 single nucleotide polymorphisms distributed across C. moschata genome. Three QTLs significantly (P < 0.05) associated with resistance to Phytophthora crown rot were detected on chromosome 4 (QtlPC-C04), 11 (QtlPC-C11) and 14 (QtlPC-C14). Several markers linked to these QTLs are potential targets for MAS against Phytophthora crown rot in C. moschata. The present study reports the first QTLs associated with Phytophthora crown rot resistance in C. moschata.
DOI: 10.1038/s41598-020-62228-z
PubMed: 32210312
PubMed Central: PMC7093484
Affiliations:
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and Education Center, University of Florida, 18905 SW 280th St, Homestead, FL, 33031, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Horticultural Sciences Department and Tropical Research and Education Center, University of Florida, 18905 SW 280th St, Homestead, FL, 33031</wicri:regionArea><wicri:noRegion>33031</wicri:noRegion></affiliation></author><author><name sortKey="Michael, Vincent" sort="Michael, Vincent" uniqKey="Michael V" first="Vincent" last="Michael">Vincent Michael</name><affiliation wicri:level="1"><nlm:affiliation>Horticultural Sciences Department and Tropical Research and Education Center, University of Florida, 18905 SW 280th St, Homestead, FL, 33031, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Horticultural Sciences Department and Tropical Research and Education Center, University of Florida, 18905 SW 280th St, Homestead, FL, 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University of Florida, 18905 SW 280th St, Homestead, FL, 33031, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Horticultural Sciences Department and Tropical Research and Education Center, University of Florida, 18905 SW 280th St, Homestead, FL, 33031</wicri:regionArea><wicri:noRegion>33031</wicri:noRegion></affiliation></author><author><name sortKey="Meru, Geoffrey" sort="Meru, Geoffrey" uniqKey="Meru G" first="Geoffrey" last="Meru">Geoffrey Meru</name><affiliation wicri:level="1"><nlm:affiliation>Horticultural Sciences Department and Tropical Research and Education Center, University of Florida, 18905 SW 280th St, Homestead, FL, 33031, USA. gmeru@ufl.edu.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Horticultural Sciences Department and Tropical Research and Education Center, University of Florida, 18905 SW 280th St, Homestead, FL, 33031</wicri:regionArea><wicri:noRegion>33031</wicri:noRegion></affiliation></author></analytic><series><title level="j">Scientific reports</title><idno type="eISSN">2045-2322</idno><imprint><date when="2020" type="published">2020</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Phytophthora capsici Leonian, the causal agent of foliar blight, root rot, fruit rot and crown rot syndromes in squash (Cucurbita moschata), is a devastating pathogen worldwide. Resistance to Phytophthora crown rot in University of Florida breeding line #394-1-27-12 (C. moschata) is conferred by three independent dominant genes (R1R2R3). Availability of DNA markers linked to R1R2R3 genes would allow efficient breeding for Phytophthora crown rot resistance through marker-assisted selection (MAS). The goal of the current study was to identify quantitative trait loci (QTLs) associated with resistance to Phytophthora crown rot in an F<sub>2</sub> population (n = 168) derived from a cross between #394-1-27-12 (R) and Butter Bush (S) using QTL-seq bulk segregant analysis. Whole-genome resequencing of the resistant (n = 20) and susceptible (n = 20) bulk segregants revealed ~900,000 single nucleotide polymorphisms distributed across C. moschata genome. Three QTLs significantly (P < 0.05) associated with resistance to Phytophthora crown rot were detected on chromosome 4 (QtlPC-C04), 11 (QtlPC-C11) and 14 (QtlPC-C14). Several markers linked to these QTLs are potential targets for MAS against Phytophthora crown rot in C. moschata. The present study reports the first QTLs associated with Phytophthora crown rot resistance in C. moschata.</div></front></TEI><pubmed><MedlineCitation Status="In-Process" Owner="NLM"><PMID Version="1">32210312</PMID><DateRevised><Year>2020</Year><Month>08</Month><Day>28</Day></DateRevised><Article PubModel="Electronic"><Journal><ISSN IssnType="Electronic">2045-2322</ISSN><JournalIssue CitedMedium="Internet"><Volume>10</Volume><Issue>1</Issue><PubDate><Year>2020</Year><Month>03</Month><Day>24</Day></PubDate></JournalIssue><Title>Scientific reports</Title><ISOAbbreviation>Sci Rep</ISOAbbreviation></Journal><ArticleTitle>QTL-seq for identification of loci associated with resistance to Phytophthora crown rot in squash.</ArticleTitle><Pagination><MedlinePgn>5326</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1038/s41598-020-62228-z</ELocationID><Abstract><AbstractText>Phytophthora capsici Leonian, the causal agent of foliar blight, root rot, fruit rot and crown rot syndromes in squash (Cucurbita moschata), is a devastating pathogen worldwide. Resistance to Phytophthora crown rot in University of Florida breeding line #394-1-27-12 (C. moschata) is conferred by three independent dominant genes (R1R2R3). Availability of DNA markers linked to R1R2R3 genes would allow efficient breeding for Phytophthora crown rot resistance through marker-assisted selection (MAS). The goal of the current study was to identify quantitative trait loci (QTLs) associated with resistance to Phytophthora crown rot in an F<sub>2</sub> population (n = 168) derived from a cross between #394-1-27-12 (R) and Butter Bush (S) using QTL-seq bulk segregant analysis. Whole-genome resequencing of the resistant (n = 20) and susceptible (n = 20) bulk segregants revealed ~900,000 single nucleotide polymorphisms distributed across C. moschata genome. Three QTLs significantly (P < 0.05) associated with resistance to Phytophthora crown rot were detected on chromosome 4 (QtlPC-C04), 11 (QtlPC-C11) and 14 (QtlPC-C14). Several markers linked to these QTLs are potential targets for MAS against Phytophthora crown rot in C. moschata. The present study reports the first QTLs associated with Phytophthora crown rot resistance in C. moschata.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Ramos</LastName><ForeName>Alexis</ForeName><Initials>A</Initials><AffiliationInfo><Affiliation>Horticultural Sciences Department and Tropical Research and Education Center, University of Florida, 18905 SW 280th St, Homestead, FL, 33031, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Fu</LastName><ForeName>Yuqing</ForeName><Initials>Y</Initials><AffiliationInfo><Affiliation>Horticultural Sciences Department and Tropical Research and Education Center, University of Florida, 18905 SW 280th St, Homestead, FL, 33031, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Michael</LastName><ForeName>Vincent</ForeName><Initials>V</Initials><AffiliationInfo><Affiliation>Horticultural Sciences Department and Tropical Research and Education Center, University of Florida, 18905 SW 280th St, Homestead, FL, 33031, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Meru</LastName><ForeName>Geoffrey</ForeName><Initials>G</Initials><AffiliationInfo><Affiliation>Horticultural Sciences Department and Tropical Research and Education Center, University of Florida, 18905 SW 280th St, Homestead, FL, 33031, USA. gmeru@ufl.edu.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><GrantList CompleteYN="Y"><Grant><GrantID>FLA-TRC-005564</GrantID><Agency>United States Department of Agriculture | National Institute of Food and Agriculture (NIFA)</Agency><Country>International</Country></Grant></GrantList><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2020</Year><Month>03</Month><Day>24</Day></ArticleDate></Article><MedlineJournalInfo><Country>England</Country><MedlineTA>Sci Rep</MedlineTA><NlmUniqueID>101563288</NlmUniqueID><ISSNLinking>2045-2322</ISSNLinking></MedlineJournalInfo><CitationSubset>IM</CitationSubset></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2019</Year><Month>09</Month><Day>05</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2020</Year><Month>02</Month><Day>28</Day></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2020</Year><Month>3</Month><Day>27</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2020</Year><Month>3</Month><Day>27</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2020</Year><Month>3</Month><Day>27</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>epublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">32210312</ArticleId><ArticleId IdType="doi">10.1038/s41598-020-62228-z</ArticleId><ArticleId IdType="pii">10.1038/s41598-020-62228-z</ArticleId><ArticleId IdType="pmc">PMC7093484</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>États-Unis</li></country></list><tree><country name="États-Unis"><noRegion><name sortKey="Ramos, Alexis" sort="Ramos, Alexis" uniqKey="Ramos A" first="Alexis" last="Ramos">Alexis Ramos</name></noRegion><name sortKey="Fu, Yuqing" sort="Fu, Yuqing" uniqKey="Fu Y" first="Yuqing" last="Fu">Yuqing Fu</name><name sortKey="Meru, Geoffrey" sort="Meru, Geoffrey" uniqKey="Meru G" first="Geoffrey" last="Meru">Geoffrey Meru</name><name sortKey="Michael, Vincent" sort="Michael, Vincent" uniqKey="Michael V" first="Vincent" last="Michael">Vincent Michael</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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